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Invitrogen
Human ISG15 ELISA Kit
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产品规格
检测灵敏度
1.64 ng/mL
测定范围
1.64-500 ng/mL
检测样本类型/体积
{Supernatant=100 µL, Plasma=100 µL, Serum=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
12%
批内差异
10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
ISG15
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Human ISG15 ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human ISG15 in serum, plasma, and cell culture supernatants.
靶标信息
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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生物信息学
基因别名 : G1P2, hUCRP, IFI15, IMD38, IP17, UCRP
基因ID : (Human) 9636
基因符号 : ISG15
蛋白别名 : 17-kDa protein, interferon, alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein, interferon-stimulated protein, 15 kDa, ubiquitin cross-reactive protein
UniProt ID (Human) P05161
