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Invitrogen
Human Insulin Rapid ELISA Kit
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产品规格
检测灵敏度
0.94 µIU/mL
测定范围
1.56-100 µIU/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Insulin
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human INS (Insulin) ELISA quantitates INS in serum and plasma.
原理方法
The Human Insulin (INS) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Insulin (INS) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Insulin (INS). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Insulin (INS) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : IDDM, IDDM1, IDDM2, ILPR, INS, IRDN, MODY10, PNDM4
基因ID : (Human) 3630
基因符号 : INS
蛋白别名 : Ins, Insulin-1, INSU1, INSU2, INSU3B, INSUA, INSUB, INSUC, Insulin, insulin splice variant, insulin variant, INS-splice, preproinsulin, proinsulin
UniProt ID (Human) P01308
