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Invitrogen
Human Luteinizing Hormone ELISA Kit
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产品规格
检测灵敏度
140 pg/mL
测定范围
140-10,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
Luteinizing Hormone
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Luteinizing Hormone (Hu LH) ELISA quantitates Hu LH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LH.
原理方法
The Human LH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
生物信息学
基因别名 : CG-ALPHA, CGB4, FSHA, GPA1, GPHa, GPHA1, HCG, HH23, LHA, LSH-B, LSH-beta, TSHA
基因ID : (Human) 1081, (Human) 3972
基因符号 : CGA, LHB
蛋白别名 : anterior pituitary glycoprotein hormones common subunit alpha, CGA, choriogonadotropin alpha chain, chorionic gonadotrophin subunit alpha, chorionic gonadotropin, alpha polypeptide, follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, follitropin alpha chain, FSH-alpha, interstitial cell stimulating hormone, beta chain, LSH-alpha, luteinizing hormone alpha chain, luteinizing hormone beta polypeptide, luteinizing hormone beta subunit, lutropin alpha chain, lutropin beta chain, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, follitropin, FSH-B, FSH-beta, hLHB, thyroid-stimulating hormone alpha chain, thyrotropin alpha chain, TSH-alpha, unnamed protein product
UniProt ID (Human) P01215, (Human) P01229
