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Invitrogen
Human MBL Oligomer ELISA Kit
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产品规格
检测灵敏度
102 pg/mL
测定范围
0.05-40 ng/mL
检测样本类型/体积
{Serum=10 µL, Plasma=10 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 15 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard (2 vials), HRP Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, TMB, Stop solution, Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
MBL2
蛋白家族
Lectin
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2.
原理方法
The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : COLEC1, HSMBPC, MBL, MBL2, MBL2D, MBP, MBP-C, MBP1, MBPD
基因ID : (Human) 4153
基因符号 : MBL2
蛋白别名 : Collectin-1, mannan-binding lectin, Mannan-binding protein, Mannose-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, Mannose-binding protein C, MBL2, MBP-C, MBP1, Collectin1, MBPC, MGC116832, MGC116833
UniProt ID (Human) P11226
