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Invitrogen
Human Marapsin/PRSS27 ELISA Kit
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产品规格
检测灵敏度
35 pg/mL
测定范围
35-10,000 pg/mL
检测样本类型/体积
{Plasma=33 µL, Serum=33 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
PRSS27
蛋白家族
Cell Structure/Organelle Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Marapsin/PRSS27 ELISA quantitates Hu Marapsin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Marapsin.
原理方法
The Human Marapsin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
PRSS27, also known as Marapsin, is a gene located on chromosome 16p13.3 that encodes a serine protease enzyme. This protein is part of the trypsin-like serine protease family, which is characterized by a catalytic triad essential for proteolytic activity. PRSS27 is primarily expressed in the epidermis and other epithelial tissues, such as the salivary glands and pancreas. It plays a role in various physiological processes, including digestion, inflammation, and immune response regulation. In the skin, PRSS27 is involved in keratinocyte differentiation and the maintenance of skin barrier function. Dysregulation or altered expression of PRSS27 has been associated with inflammatory skin conditions, such as psoriasis, where it may influence the inflammatory cascade and contribute to disease pathogenesis. Research into PRSS27 continues to explore its potential role in epithelial homeostasis and as a therapeutic target for skin-related and other inflammatory disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CAPH2, MPN, PRSS27, UNQ1884/PRO4327
基因ID : (Human) 83886
基因符号 : PRSS27
蛋白别名 : channel-activating protease 2, Marapsin, Pancreasin, CAP-2, EC 3.4.21, EC 3.4.21.4, EC 3.4.21.43, MPNMarapsin, Serine protease 27
UniProt ID (Human) Q9BQR3
