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Invitrogen
Human Melatonin ELISA Kit
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产品规格
检测灵敏度
9.38 pg/mL
测定范围
15.63-1,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Melatonin
蛋白家族
Neurotransmitter
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Melatonin (MT) ELISA quantitates MT in serum, plasma and other biological fluids
原理方法:
The Competitive Melatonin (MT) ELISA research-use-only kit is designed to quantitatively measure MT in Humans. A MT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human MT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human MT in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Melatonin is a hormone primarily released by the pineal gland at night, and has long been associated with control of the sleep-wake cycle. In vertebrates, melatonin is involved in synchronizing circadian rhythms, including sleep-wake timing and blood pressure regulation, and controls seasonal rhythmicity including reproduction, fattening, moulting, and hibernation
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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