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Invitrogen
Human/Mouse XIAP ELISA Kit
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产品规格
检测灵敏度
250 pg/mL
测定范围
0.25-600 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
XIAP
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human XIAP ELISA quantitates Hu XIAP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu XIAP.
原理方法
The Human XIAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : 1110015C02Rik, Aipa, API3, BIRC4, hIAP-3, hIAP3, IAP-3, IAP3, ILP-1, ILP1, MIHA, XIAP, XLP2
基因ID : (Human) 331, (Mouse) 11798
基因符号 : XIAP, Xiap
蛋白别名 : Baculoviral IAP repeat-containing protein 4, BIR4, HILP, RP1-315G1.5, E3 ubiquitin-protein ligase XIAP, IAP homolog A, IAP-3, IAP-like protein, ILP, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, X-linked IAP, X-linked inhibitor of apoptosis protein, X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
UniProt ID (Human) P98170, (Mouse) Q60989
