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Invitrogen
Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit
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![Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/650/NF-kB-phospho-v2-TDS.jpg?time=20240507 "Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit")
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Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit
Representative data for NFkB p65 (Phospho) [pS536] Multispecies InstantOne™ ELISA. HeLa cells were unstimulated (-) or stimulated with 10 ng/mL TNF alpha for 5 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 65 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
![Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/150/NF-kB-phospho-v2-TDS.jpg?time=20240507)
![Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit (85-86082-11)](https://www.thermofisher.cn/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
NFkB p65
Target site-specificity
Phosphorylated Ser536
均相（无需洗涤）
Human
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : NFKB3, p65, RELA
基因ID : (Human) 5970
基因符号 : RELA
蛋白别名 : EBP-1, NFKB1, NFkB-p50, nuclear factor, nuclear factor kappa b, REL A, MGC131774, NF KB, NFkappaB p65, nf-kappa-b p65, NF-kB p65, nfkb rela, NF-kappa-B p65delta3, NF-kappa-B transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Transcription factor p65, v-rel avian reticuloendotheliosis viral oncogene homolog A, v-rel reticuloendotheliosis viral oncogene homolog A
UniProt ID (Human) Q04206
