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产品规格
检测灵敏度
Lot-specific
测定范围
Lot-specific
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
This Human NGF ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
NGF
蛋白家族
Growth Factors & Receptors
均相（无需洗涤）
Human
检测试剂盒格式
ELISA Development Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
900-K60K consists of: 900-K60 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human NGF ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human NGF in a sandwich ELISA format. Human NGF ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
靶标信息
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : Beta-NGF, HSAN5, NGF, NGFB
基因ID : (Human) 4803
基因符号 : NGF
蛋白别名 : B nerve growth factor, beta ngf, BetaNGF, bNGF, b-NGF, MGC161426, β nerve growth factor, β NGF, βNGF, MGC161428, Nerve growth factor, NGF b, NGF β, NGFbeta, NGFβ, Beta-nerve growth factor, Beta-NGF, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit
UniProt ID (Human) P01138
