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Invitrogen
Human Osteoprotegerin Rapid ELISA Kit
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产品规格
检测灵敏度
0.28 ng/mL
测定范围
0.47-30 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TNFRSF11B
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human OPG (Osteoprotegerin) ELISA quantitates OPG in serum and plasma.
原理方法
The Human Osteoprotegerin (OPG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Osteoprotegerin (OPG) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Osteoprotegerin (OPG). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Osteoprotegerin (OPG) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : OCIF, OPG, PDB5, TNFRSF11B, TR1
基因ID : (Human) 4982
基因符号 : TNFRSF11B
蛋白别名 : MGC29565, soluble OPG, soluble Osteoprotegerin, sOPG, OPG, Osteoclastogenesis inhibitory factor, Osteoprotegerin, Tumor necrosis factor receptor superfamily member 11B, tumor necrosis factor receptor superfamily, member 11b, unnamed protein product
UniProt ID (Human) O00300
