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Invitrogen
Human PARP (Cleaved) [214/215] ELISA Kit
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![Human PARP (Cleaved) [214/215] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/KHO0741_SC_080404.jpg?time=20220826 "Human PARP (Cleaved) [214/215] ELISA Kit")
![Human PARP (Cleaved) [214/215] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHO0741_SC_080404.jpg?time=20220826)
![Human PARP (Cleaved) [214/215] ELISA Kit (KHO0741)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
产品规格
检测灵敏度
<0.062 ng/mL
测定范围
0.156-10 ng/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
5.3%
批内差异
4.6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PARP1 (cleaved Asp214)
Target sub-specificity
Cleaved
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human PARP (Cleaved) [214/215] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of PARP (Cleaved) [214/215] ) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PARP (Cleaved) [214/215].
原理方法
A monoclonal capture antibody specific for PARP (Cleaved) [214/215] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : ADPRT, ADPRT 1, ADPRT1, ARTD1, pADPRT-1, PARP, PARP-1, PARP1, PPOL
基因ID : (Human) 142
基因符号 : PARP1
蛋白别名 : ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase), ADP-ribosyltransferase diphtheria toxin-like 1, ADP-ribosyltransferase NAD(+), ADPRT 1, ARTD1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, PARP-1, poly (ADP-ribose) polymerase 1, poly (ADP-ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, poly(ADP-ribose) polymerase, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1
UniProt ID (Human) P09874
