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Invitrogen
Human PIIINP Rapid ELISA Kit
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产品规格
检测灵敏度
0.09 ng/mL
测定范围
0.16-10 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Procollagen III
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human PIIINP (N-Terminal Procollagen III Propeptide) ELISA quantitates PIIINP in serum and plasma.
原理方法
The Human N-Terminal Procollagen III Propeptide (PIIINP) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of N-Terminal Procollagen III Propeptide (PIIINP) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to N-Terminal Procollagen III Propeptide (PIIINP). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of N-Terminal Procollagen III Propeptide (PIIINP) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Collagen type III is synthesized as a homo-trimeric pro-collagen (Procollagen III) comprising three identical pro-alpha(III)-chains. It has been reported that procollagen type III is processed extracellularly at the ECM and can be found by immunostaining intracellular as well as extracellular. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : COL3A1, EDS4A, EDSVASC, PMGEDSV
基因ID : (Human) 1281
基因符号 : COL3A1
蛋白别名 : alpha-1 type III collagen, alpha-1(III) procollagen, alpha1 (III) collagen, Collagen alpha-1(III) chain, collagen, fetal, type III, alpha 1, Ehlers-Danlos syndrome type IV, autosomal dominant, unnamed protein product
UniProt ID (Human) P02461
