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Invitrogen
Human PLAP/ALPP Rapid ELISA Kit
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产品规格
检测灵敏度
46.88 pg/mL
测定范围
78.13-5000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Placental Alkaline Phosphatase
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA quantitates PLAP/ALPP in serum and plasma.
原理方法
The Human Placental Alkaline Phosphatase (PLAP/ALPP) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Placental Alkaline Phosphatase (PLAP/ALPP) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Placental Alkaline Phosphatase (PLAP/ALPP). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Placental Alkaline Phosphatase (PLAP/ALPP) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Placental Alkaline Phosphatase plays an important role in the regulation of specific inflammatory disease processes. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney for this form of alkaline phosphatase have been well characterized. Placental Alkaline Phosphatase reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. Placental Alkaline Phosphatase is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). A proposed function of Placental Alkaline Phosphatase is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. Placental Alkaline Phosphatase has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of hypophosphatasia can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ALP, ALPI, ALPP, IAP, PALP, PLAP, PLAP-1
基因ID : (Human) 250
基因符号 : ALPP
蛋白别名 : Alkaline phosphatase placental type, Alp1, AP-TNAP, DOA1, FLJ11281, FLJ40094, Regan isozyme, TNAP, TNSALP, nagao Isozyme, P PLAP, PLA2P, PLAA, placental heat-stable alkaline phosphatase, PLAP1, FLJ61142, FLJ93059, Germ-cell alkaline phosphatase, HOPS, MGC161443, MGC167935, Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type, alkaline phosphomonoesterase, glycerophosphatase, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase, Placental alkaline phosphatase 1, placental alkaline phosphatase precursor, PLAP-1, preplacental alkaline phosphatase (EC 3.1.3.1), preplacental alkaline phosphatase type 1 (EC 3.1.3.1), unnamed protein product
UniProt ID (Human) P05187
