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Invitrogen
Human RNASE3 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
23.44 pg/mL
Assay range
39.06-2500 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
RNASE3
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human RNASE3 (Ribonuclease A3/Eosinophil Cationic Protein) ELISA quantitates RNASE3 in serum and plasma.
Principle of the method
The Human Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Ribonuclease A3/Eosinophil Cationic Protein (RNASE3). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
ECP (eosinophil cationic protein), also known as RNS3 or RNASE3 (ribonuclease, RNase A family, 3), is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ECP, RAF1, RNASE3, RNS3
Gene ID : (Human) 6037
Gene symbol : RNASE3
Protein Aliases : cytotoxic ribonuclease, ECP, Eosinophil cationic protein, Ribonuclease 3, ribonuclease, RNase A family, 3, RNase 3, unnamed protein product
UniProt ID (Human) P12724
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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