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Invitrogen
Human SARS-CoV-2 N ELISA Kit
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产品规格
检测灵敏度
0.069 ng/mL
测定范围
0.069-50 ng/mL
检测样本类型/体积
{Serum=25 µL, Plasma=25 µL, Supernatant=25 µL, Nasopharyngeal Swab=25 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
SARS Coronavirus Nucleocapsid
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human SARS-CoV-2 Nucleocapsid ELISA quantitates Nucleocapsid in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Human Nucleocapsid.
原理方法
The Human SARS-CoV-2 Nucleocapsid solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : Coronavirus, COVID, COVID 19, COVID19, COVID-19, SARS, SARS-CoV-2, SARS Coronavirus, SARS CoV, SARS CoV2, SARS CoV-2, SARS Nucleocapsid, SARS-CoV2
