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Invitrogen
Human SMAD4 ELISA Kit
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产品规格
检测灵敏度
1.3 ng/mL
测定范围
1.3-300 ng/mL
检测样本类型/体积
{Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
SMAD4
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Human SMAD4 quantitates human SMAD4 in supernatant. The assay will exclusively recognize both natural and recombinant human SMAD4.
靶标信息
Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : DPC4, JIP, MADH4, MYHRS, SMAD4
基因ID : (Human) 4089
基因符号 : SMAD4
蛋白别名 : Deleted in Pancreatic Carcinoma 4 (DPC4), hSMAD4, MAD homolog 4 (MADH4), Mothers against decapentaplegic homolog 4 (MADH4), SMAD, deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, Mothers against decapentaplegic homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4, SMAD 4, SMAD family member 4, mothers against DPP homolog 4
UniProt ID (Human) Q13485
