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Invitrogen
Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit
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![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/650/stat1-phos-tds.jpg?time=20240507 "Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit")
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Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit
Representative data for STAT1 (Phospho) [pY701] Multispecies InstantOne™ ELISA. A431 cells were either unstimulated (-) or treated with EGF (1 µg/mL for 10 min) (+). Lysates were prepared as described in the InstantOne™ User Manual. Phosphorylated STAT1 (Tyr701) was analyzed in cell lysates by immunoblotting (left) and InstantOne™ ELISA (right) 5 µg/analysis. The intensity of the band as detected by immuno... View More
![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/150/stat1-phos-tds.jpg?time=20240507)
![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit (85-86092-11)](https://www.thermofisher.cn/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
STAT1
Target site-specificity
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT1 (Tyr701) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately two hours. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Transcription factor ISGF-3 components p91/p84
