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Invitrogen
Human TFF-3 Rapid ELISA Kit
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产品规格
检测灵敏度
23.44 pg/mL
测定范围
39.06-2500 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TFF3
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human TFF3 (Trefoil Factor 3, Intestinal) ELISA quantitates TFF3 in serum and plasma.
原理方法
The Human Trefoil Factor 3, Intestinal (TFF3) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Trefoil Factor 3, Intestinal (TFF3) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Trefoil Factor 3, Intestinal (TFF3). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Trefoil Factor 3, Intestinal (TFF3) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ITF, P1B, TFF3, TFI
基因ID : (Human) 7033
基因符号 : TFF3
蛋白别名 : hITF, hP1.B, Intestinal trefoil factor, Polypeptide P1.B, TFF3, Trefoil factor 3, trefoil factor 3 (intestinal)
UniProt ID (Human) Q07654
