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Invitrogen
Human TFPI Rapid ELISA Kit
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产品规格
检测灵敏度
37.51 pg/mL
测定范围
62.5-4000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TFPI
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human TFPI (Tissue Factor Pathway Inhibitor) ELISA quantitates TFPI in serum and plasma.
原理方法
The Human Tissue Factor Pathway Inhibitor (TFPI) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Tissue Factor Pathway Inhibitor (TFPI) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Tissue Factor Pathway Inhibitor (TFPI) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Tissue factor pathway inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor, is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : EPI, LACI, TFI, TFPI, TFPI1
基因ID : (Human) 7035
基因符号 : TFPI
蛋白别名 : anti-convertin, EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor, lipoprotein-associated coagulation inhibitor precursor, OTTHUMP00000205431, OTTHUMP00000163472, TFPI, Tissue factor pathway inhibitor, tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), unnamed protein product
UniProt ID (Human) P10646
