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Invitrogen
Human TGF beta-1 (LAP) ELISA Kit
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产品规格
检测灵敏度
0.098 ng/mL
测定范围
0.16-10 ng/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
6%
批内差异
7.6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
TGF beta-1
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Transforming Growth Factor Beta 1/Latency Associated Peptide (Hu TGFβ1/LAP) ELISA quantitates Hu TGFβ1/LAP in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβ1/LAP.
原理方法
The Human TGFβ1/LAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CED, DPD1, LAP, TGFB, TGFB1, TGFbeta
基因ID : (Human) 7040
基因符号 : TGFB1
蛋白别名 : latency-associated peptide, prepro-transforming growth factor beta-1, TGF-beta-1, LAP, TGF b, TGF b 1, transforming growth factor b1, transforming growth factor beta, transforming growth factor β1, TGF β 1, TGF β1, TGF-B1, TGF-beta 1 protein, TGFβ1, Transforming growth factor, TGF beta, tgf beta 1, tgf beta1, TGF beta-1, TGF beta-1 protein, TGF β, Transforming growth factor beta-1 proprotein
UniProt ID (Human) P01137
