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The Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA quantitates TMPRSS2 in serum, plasma, and other biological fluids
The Human Transmembrane Protease, Serine 2 (TMPRSS2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. Its gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : PP9284, PRSS10, TMPRSS2
基因ID : (Human) 7113
基因符号 : TMPRSS2
蛋白别名 : EC 3.4.219, FLJ41954, MGC6821, TMPS2, epitheliasin, Serine protease 10, Transmembrane protease serine 2
UniProt ID (Human) O15393