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Invitrogen
Human TNFR1 Rapid ELISA Kit
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产品规格
检测灵敏度
0.09 ng/mL
测定范围
0.16-10 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TNFR1
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily;Member 1A) ELISA quantitates TNFRSF1A in serum and plasma.
原理方法
The Human Tumor Necrosis Factor Receptor Superfamily;Member 1A (TNFRSF1A) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Tumor Necrosis Factor Receptor Superfamily;Member 1A (TNFRSF1A) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Tumor Necrosis Factor Receptor Superfamily;Member 1A (TNFRSF1A). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Tumor Necrosis Factor Receptor Superfamily;Member 1A (TNFRSF1A) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CD120a, FPF, p55, p55-R, p60, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, TNFRSF1A
基因ID : (Human) 7132
基因符号 : TNFRSF1A
蛋白别名 : CD120a, CD120a antigen, MGC19588, n-smase activation assoc. factor, p62, p63, sCD120a, TNFR, TNFR I, Tumor necrosis factor receptor, soluble CD120a, soluble TNFR1, sTNF RI, sTNFR I, sTNFR1, sTNFRI, p55, p60, TNF alpha gene, TNF-R1, TNF-RI, TNFR-I, TNFR1 beta, tumor necrosis factor binding protein 1, tumor necrosis factor receptor, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor receptor type 1, Tumor necrosis factor receptor type I, tumor necrosis factor-alpha receptor, unnamed protein product
UniProt ID (Human) P19438
