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Invitrogen
Human TNFRSF10B (DR5) ELISA Kit
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产品规格
检测灵敏度
2 pg/mL
测定范围
2-600 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
TRAIL-R2 (DR5)
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Tumor Necrosis Factor Receptor SuperFamily 10 B/Death Receptor 5 (TNFRSF10B (DR5)) ELISA quantitates Hu DR5 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DR5.
原理方法
The Human DR5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CD262, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, UNQ160/PRO186, ZTNFR9
基因ID : (Human) 8795
基因符号 : TNFRSF10B
蛋白别名 : apoptosis inducing protein TRICK2A/2B, apoptosis inducing receptor TRAIL-R2, CD262, cytotoxic TRAIL receptor-2, death domain containing receptor for TRAIL/Apo-2L, Death receptor 5, dr-5, sCD262, soluble CD262, Fas-like protein, p53-regulated DNA damage-inducible cell death receptor(killer), TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, Tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9
UniProt ID (Human) O14763
