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Invitrogen
Human TNFRSF9 ELISA Kit
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产品规格
检测灵敏度
10 pg/mL
测定范围
10-3,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
4-1BB (CD137)
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human TNFRSF9 ELISA quantitates Hu TNFRSF9 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFRSF9.
原理方法
The Human TNFRSF9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : 4-1BB, CD137, CDw137, ILA, TNFRSF9
基因ID : (Human) 3604
基因符号 : TNFRSF9
蛋白别名 : 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, 4 1 BB, 41BB, s4 1 BB, s4 1BB, s41BB, sCD137, soluble 4 1 BB, soluble 4 1BB, soluble 41BB, soluble CD137, CD137, CD137 antigen, homolog of mouse 4-1BB, induced by lymphocyte activation (ILA), interleukin-activated receptor, homolog of mouse Ly63, receptor protein 4-1BB, T cell antigen ILA, Tumor necrosis factor receptor superfamily member 9, tumor necrosis factor receptor superfamily, member 9
UniProt ID (Human) Q07011
