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![Human Tau (Phospho) [pT181] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/KHO0631_SC_080327.jpg?time=20240507 "Human Tau (Phospho) [pT181] ELISA Kit")
![Human Tau (Phospho) [pT181] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHO0631_SC_080327.jpg?time=20240507)
![Human Tau (Phospho) [pT181] ELISA Kit (KHO0631)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20240507)
产品规格
检测灵敏度
<10 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Cerebrospinal Fluid=10 µL, Supernatant=10 µL, Tissue Homogenate=10 µL}
手工操作时间
1 hr 20 min
总时间
15 hr
均相（无需洗涤）
No
批间差异
5.7%
批内差异
4.3%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Tau
Target site-specificity
Phosphorylated Thr181
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Tau (Phospho) [pT181] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of Tau (Phospho) [pT181] in human cerebrospinal fluid, tissue homogenates, buffered solutions and cell culture supernatants. The assay recognizes both natural and recombinant Tau (Phospho) [pT181].
原理方法
A monoclonal capture antibody specific for Tau (Phospho) [pT181] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : DDPAC, FTDP-17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU
基因ID : (Human) 4137
基因符号 : MAPT
蛋白别名 : G protein beta1/gamma2 subunit-interacting factor 1, Microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, FLJ31424, FTDP17, Tau-4, Tau5, map tau, MGC138549, microtubules, neurofibrillary tangles, Neuronal Marker, PHFtau, protein phosphatase 1, regulatory subunit 103
UniProt ID (Human) P10636
