Search
Invitrogen
Human Trappin-2/PI3 ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
产品规格
检测灵敏度
5 pg/mL
测定范围
5-5,000 pg/mL
检测样本类型/体积
{Plasma=3.3 µL, Serum=3.3 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
PI3
蛋白家族
Proteases Peptidases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Trappin-2/PI3 ELISA quantitates Hu PI3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PI3.
原理方法
The Human PI3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Peptidase inhibitor 3 (PI3), also known as Elafin, is a gene located on chromosome 20q13.12, encoding a protein that functions as a serine protease inhibitor. Elafin is primarily expressed in epithelial tissues, including skin and mucosal surfaces, where it plays a protective role against excessive proteolytic activity by inhibiting enzymes like neutrophil elastase and proteinase 3. This activity is crucial for maintaining tissue homeostasis and preventing inflammatory damage. PI3 is regulated by pro-inflammatory cytokines and is involved in modulating inflammatory responses and tissue repair processes. Its expression is often elevated in conditions characterized by inflammation, such as chronic obstructive pulmonary disease (COPD), psoriasis, and other inflammatory diseases, reflecting its role in guarding against protease-induced tissue damage. Research into PI3 focuses on its potential therapeutic applications in inflammatory diseases and its utility as a biomarker for assessing inflammation-related pathologies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : cementoin, ESI, PI3, SKALP, WAP3, WFDC14
基因ID : (Human) 5266
基因符号 : PI3
蛋白别名 : Elafin, Elastase-specific inhibitor, ESI, Peptidase inhibitor 3, peptidase inhibitor 3, skin-derived, PI-3, pre-elafin, protease inhibitor 3, skin-derived (SKALP), Protease inhibitor WAP3, SKALP, Skin-derived antileukoproteinase, trappin-2, WAP four-disulfide core domain 14, WAP four-disulfide core domain protein 14
UniProt ID (Human) P19957
