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Invitrogen
Human VCAM-1 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.47 ng/mL
Assay range
0.78-50 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
VCAM-1 (CD106)
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA quantitates VCAM-1 in serum and plasma.
Principle of the method
The Human Vascular Cell Adhesion Molecule 1 (VCAM-1/CD106) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Vascular Cell Adhesion Molecule 1 (VCAM-1/CD106) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM-1/CD106). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Vascular Cell Adhesion Molecule 1 (VCAM-1/CD106) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD106, INCAM-100, VCAM1
Gene ID : (Human) 7412
Gene symbol : VCAM1
Protein Aliases : CD106, CD106 antigen, DKFZp779G2333, INCAM100, MGC99561, sCD106, soluble CD106, soluble VCAM 1, sVCAM 1, INCAM-100, unnamed protein product, V-CAM 1, vascular cell adhesion molecule 1 precursor, Vascular cell adhesion protein 1
UniProt ID (Human) P19320
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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