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Invitrogen
Human VWF Rapid ELISA Kit
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产品规格
检测灵敏度
0.23 ng/mL
测定范围
0.39-25 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
VWF
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human VWF (Von Willebrand Factor) ELISA quantitates VWF in serum and plasma.
原理方法
The Human Von Willebrand Factor (VWF) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Von Willebrand Factor (VWF) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Von Willebrand Factor (VWF). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Von Willebrand Factor (VWF) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : F8VWF, VWD, VWF
基因ID : (Human) 7450
基因符号 : VWF
蛋白别名 : coagulation factor VIII VWF, Factor VIII related antigen, factor viii related antigen, von Willebrand Disease (vWD), Coagulation Factor VIII, unnamed protein product, von Willebrand antigen II precursor, von Willebrand factor, vWF
UniProt ID (Human) P04275
