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Invitrogen
Human hCG Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
4.69 mIU/mL
Assay range
7.81-500 mIU/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
hCG
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human HCG (Chorionic Gonadotropin) ELISA quantitates HCG in serum and plasma.
Principle of the method
The Human Chorionic Gonadotropin (HCG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Chorionic Gonadotropin (HCG) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Chorionic Gonadotropin (HCG). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Chorionic Gonadotropin (HCG) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CG-ALPHA, CGA, CGB, CGB3, CGB5, CGB7, CGB8, FSHA, GPA1, GPHa, GPHA1, HCG, hCGB, LHA, LHB, TSHA
Gene ID : (Human) 1081, (Human) 1082, (Human) 93659, (Human) 94115
Gene symbol : CGA, CGB3, CGB5, CGB8
Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, CG-alpha, CG-beta, CGA, Choriogonadotropin alpha chain, Choriogonadotropin subunit beta, Choriogonadotropin subunit beta 3, chorionic gonadotrophin chain beta, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta 5 subunit, chorionic gonadotropin beta 8 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit 3, chorionic gonadotropin beta subunit 5, chorionic gonadotropin beta subunit 8, Chorionic gonadotropin chain beta, chorionic gonadotropin human, human chorionic gonadotropin, chorionic gonadotropin, alpha polypeptide, beta polypeptide, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, LSH-alpha, Luteinizing hormone alpha chain, luteinizing hormone beta subunit, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha, unnamed protein product
UniProt ID (Human) P01215, (Human) P0DN86, (Human) P0DN86, (Human) P0DN86
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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