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Invitrogen
Human p38 MAPK (Total) ELISA Kit
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产品规格
检测灵敏度
<16 pg/mL
测定范围
31.2-2,000 pg/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
7.3%
批内差异
5.1%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
p38 MAPK alpha
Target sub-specificity
Total
蛋白亚型
Serine/Threonine Kinases
蛋白家族
Kinases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human p38 MAPK (Total) ELISA quantitates Hu p38 MAPK (Total) in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu p38 MAPK (Total).
原理方法
The Human p38 MAPK (Total) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAPK14, MXI2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A
基因ID : (Human) 1432
基因符号 : MAPK14
蛋白别名 : CSAID-binding protein, Csaids binding protein, cytokine suppressive anti-inflammatory drug binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EC 2.7.11.24, ERK1, ERT2, MAP 14, MAP kinase 1, RP1-179N16.5, MAP kinase p38, MAP kinase p38alpha, MAPK1 antibody, MK14, OTTHUMP00000216563, OTTHUMP00000216566, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAX-interacting protein 2, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, SAPK2a, Stress-activated protein kinase 2a
UniProt ID (Human) Q16539
