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Invitrogen
IP3 Competitive ELISA Kit
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产品规格
检测灵敏度
9.38 pg/mL
测定范围
15.63-1,000 pg/mL
检测样本类型/体积
{Serum=100 µL, Plasma=100 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
IP3
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
IP3 Competitive ELISA Kit quantifies IP3 in serum and plasma.
The IP3 Competitive ELISA research-use-only kit is designed to quantitatively measure IP3. A IP3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to IP3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve..
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Inositol 1,4,5-trisphosphate (IP3) is a crucial intracellular signaling molecule that plays an essential role in various cellular processes by regulating calcium release from the endoplasmic reticulum. IP3 is generated through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by the enzyme phospholipase C (PLC) following receptor activation. The released IP3 binds to its receptors, inositol 1,4,5-trisphosphate receptors (ITPRs), which are located on the endoplasmic reticulum membrane. This binding triggers the release of Ca2+ ions into the cytoplasm, leading to numerous downstream effects, including muscle contraction, secretion, metabolism, and cell growth. ITPRs are encoded by different genes leading to multiple isoforms (ITPR1, ITPR2, and ITPR3), each showing distinct tissue distribution and regulatory properties. Understanding the specific functions and regulatory mechanisms of IP3 and ITPRs is critical for elucidating their roles in health and disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Inositol Triphosphate, Inositol 1, 4, 5-trisphosphate
