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Invitrogen
In-Cell ELISA Kit, Near Infrared
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In-Cell ELISA Kit, Near Infrared
A431 cells were untreated or treated with 100 ng/mL EGF(green) or both 100 ng/mL EGF and 25 nM PD168393(purple)and measurements of pSTAT3, pSTAT6 and pEGFR were performed using the In-Cell ELISA Colorimetric Detection Kit. Results show incomplete inhibition of phosphorylation when cells are treated with PD168393.
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产品规格
检测样本类型/体积
{Supernatant=10,000 cells}
手工操作时间
1 hr 20 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Fluorescent Microplate Reader
产品规格
4 x 96 Tests
产品成分
Blocking Buffer
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
运输条件
Wet ice
储存
2-8°C
蛋白名称
Intracellular Proteins
Target site-specificity
蛋白家族
Other Proteins
检测试剂盒格式
In-cell ELISA Kit
检测抗体结合物
Unconjugated
标签或染料
DyLight™ 680 , DyLight™ 800
关于本试剂盒
The In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells.
原理方法
This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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