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Invitrogen
Mouse ACTA2 ELISA Kit
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产品规格
检测灵敏度
1.88 ng/mL
测定范围
3.13-200 ng/mL
检测样本类型/体积
{Serum=100 µL, Tissue Homogenate=100 µL, Cell Lysate=100 µL, Plasma=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Alpha-Smooth Muscle Actin
均相（无需洗涤）
Mouse
Species (reported)
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Actin Alpha 2, Smooth Muscle (ACTalpha2) ELISA quantitates ACTalpha2 in serum, plasma and other biological fluids.
原理方法
The Mouse Actin Alpha 2, Smooth Muscle (ACTa2) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : 0610041G09Rik, a-SMA, Acta2, Actsa, Actvs, alphaSMA, SMalphaA
基因ID : (Mouse) 11475
基因符号 : Acta2
蛋白别名 : Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, Alpha Cardiac Actin, Aortic Smooth Muscle, asma, Growth Inhibiting Gene 46, smooth muscle, actin, alpha, vascular smooth muscle, Actin, aortic smooth muscle, Alpha-actin-2
UniProt ID (Mouse) P62737
