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Invitrogen
Mouse CD80 Rapid ELISA Kit
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产品规格
检测灵敏度
0.23 ng/mL
测定范围
0.39-25 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
CD80 (B7-1)
均相（无需洗涤）
Mouse
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Mouse LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA quantitates LAB7-1 in serum and plasma.
原理方法
The Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of B-Lymphocyte Activation Antigen B7-1 (LAB7-1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to B-Lymphocyte Activation Antigen B7-1 (LAB7-1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of B-Lymphocyte Activation Antigen B7-1 (LAB7-1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : B7, B71, Cd28l, Cd80, Ly-53, Ly53, MIC17, TSA1
基因ID : (Mouse) 12519
基因符号 : Cd80
蛋白别名 : Activation B7-1 antigen, B7, B7 protein, CD80, sCD 80, sCD80, soluble CD 80, soluble CD80, TS/A-1, T-lymphocyte activation antigen CD80
UniProt ID (Mouse) Q00609
