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Invitrogen
Mouse HO-1 ELISA Kit
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产品规格
检测灵敏度
37.5 pg/mL
测定范围
62.5-4,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Tissue Homogenate=100 µL, Cell Lysate=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
HO-1
均相（无需洗涤）
Mouse
Species (reported)
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Heme Oxygenase 1 (HO1) ELISA quantitates HO1 in serum, plasma and other biological fluids.
原理方法
The Mouse Heme Oxygenase 1 (HO1) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : D8Wsu38e, Hemox, Hmox, Hmox1, HO-1, HO1, Hsp32
基因ID : (Mouse) 15368
基因符号 : Hmox1
蛋白别名 : heme orygenase 1, heme oxygenase (decycling) 1, Heme oxygenase 1, hemoxygenase, HO-1, P32 protein
UniProt ID (Mouse) P14901
