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Invitrogen
Mouse IL-17E/IL-25 Uncoated ELISA Kit with Plates
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产品规格
检测灵敏度
32 pg/mL
测定范围
32-2,000 pg/mL
检测样本类型/体积
{Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
2 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Sample Diluent B: 12 mL of a 1X solution per plate
Detection Enzyme: Pre-titrated Streptavidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Sample Diluent B: 12 mL of a 1X solution per plate
Detection Enzyme: Pre-titrated Streptavidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-25
均相（无需洗涤）
Mouse
检测试剂盒格式
Uncoated ELISA Kit with Plates
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Interleukin 17E/Interleukin 25 (IL-17E) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17E. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : IL-17e, Il17e, Il25
基因ID : (Mouse) 140806
基因符号 : Il25
蛋白别名 : il 17e, ILN, Interleukin, IL-17E, IL-25, interleukin 17E, Interleukin-17E, Interleukin-25
UniProt ID (Mouse) Q8VHH8
