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The Mouse IP-10 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample dilutent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IP-10. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : C7, CRG-2, Crg2, Cxcl10, gIP-10, Ifi10, INP10, IP-10, IP10, mob-1, Scyb10
基因ID : (Mouse) 15945
基因符号 : Cxcl10
蛋白别名 : 10 kDa interferon gamma-induced protein, C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, CXC motif chemokine, CXCL, H-IP-10, M-IP-10, C-X-C motif chemokine 10, C7, Gamma-IP10, interferon activated gene 10, Interferon-gamma induced protein CRG-2, interferon-gamma-induced protein CRG-2, small inducible cytokine B subfamily (Cys-X-Cys), member 10, Small-inducible cytokine B10
UniProt ID (Mouse) P17515