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Invitrogen
Mouse MFG-E8 ELISA Kit
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产品规格
检测灵敏度
1 pg/mL
测定范围
1-600 pg/mL
检测样本类型/体积
{Plasma=20 µL, Serum=20 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
MFGE8 (Lactadherin)
蛋白家族
Proteases Peptidases & Inhibitors
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse MFG-E8 ELISA quantitates Ms MFG-E8 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MFG-E8.
原理方法
The Mouse MFG-E8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : AA408458, AI325141, lactadherin, MFG-E8, Mfge8, Mfgm, MP47, P47, SED1
基因ID : (Mouse) 17304
基因符号 : Mfge8
蛋白别名 : EGF/factor VIII, Lactadherin, MFG-E8, MFGM, Milk fat globule-EGF factor 8, SED1, Sperm surface protein SP47, MP47, milk fat globule membrane, milk fat globule membrane protein, milk fat globule glycoprotein
UniProt ID (Mouse) P21956
