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Invitrogen
Mouse PRDC (GREM2) ELISA Kit
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产品规格
检测灵敏度
3 pg/mL
测定范围
3.28-800 pg/mL
检测样本类型/体积
{Plasma=33 µL, Serum=33 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
GREM2
蛋白家族
Other Proteins
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse PRDC (GREM2) ELISA quantitates Ms PDRC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PDRC.
原理方法
The Mouse PDRC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
GREM2 encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Cktsf1b2, Grem2, Gremlin2, Prdc
基因ID : (Mouse) 23893
基因符号 : Grem2
蛋白别名 : Cysteine knot superfamily 1, BMP antagonist 2, Protein related to DAN and cerberus, PRDC, CKTSF1B2FLJ21195, DAND3Prdc, PRDCgremlin 2, Gremlin-2, Gremlin1
UniProt ID (Mouse) O88273
