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Invitrogen
Mouse ST2 (IL-33R) (Soluble) Rapid ELISA Kit
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产品规格
检测灵敏度
0.19 ng/mL
测定范围
0.31-20 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
ST2
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human sST2 (Soluble ST2) ELISA quantitates sST2 in serum and plasma.
原理方法
The Human Soluble ST2 (sST2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Soluble ST2 (sST2) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Soluble ST2 (sST2). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Soluble ST2 (sST2) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : DER4, FIT-1, IL1RL1, IL33R, ST2, ST2L, ST2V, T1
基因ID : (Human) 9173
基因符号 : IL1RL1
蛋白别名 : growth stimulation-expressed, homolog of mouse growth stimulation-expressed, IL-1 R4, IL-1RL1, IL-33R, Interleukin1 receptor like 1, sIL 33R, soluble IL 33 Receptor, soluble IL 33R, interleukin 1 receptor-related protein, Interleukin-1 receptor-like 1, Protein ST2, soluble protein receptor, unnamed protein product
UniProt ID (Human) Q01638
