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Invitrogen
Mouse TRACP/PAP/ACP5 Rapid ELISA Kit
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产品规格
检测灵敏度
46.88 pg/mL
测定范围
78.13-5000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Tartrate Resistant Acid Phosphatase
均相（无需洗涤）
Mouse
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5) ELISA quantitates TRACP-5b in serum and plasma.
原理方法
The Mouse Tartrate Resistant Acid Phosphatase 5 (TRACP-5b) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Tartrate Resistant Acid Phosphatase 5 (TRACP-5b) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Tartrate Resistant Acid Phosphatase 5 (TRACP-5b). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Tartrate Resistant Acid Phosphatase 5 (TRACP-5b) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression is increased in spleen and monocytes of patients with Gaucher's disease; spleenocytes and circulating leukocytes of patients with hairy cell leukemia; spleen of patients with Hodgkin's lymphoma and in the sera of patients undergoing active bone turnover.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Acp5, T5ap, TRACP, TRAP
基因ID : (Mouse) 11433
基因符号 : Acp5
蛋白别名 : acid phosphatase type V, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP, TRAPase, TrATPase, Type 5 acid phosphatase
UniProt ID (Mouse) Q05117
