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Invitrogen
Mouse Th1/Th2 Uncoated ELISA Kit
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产品规格
检测灵敏度
IFN-gamma: 15 pg/mL; IL-2: 2 pg/mL; IL-4: 4 pg/mL; IL-10: 30 pg/mL
测定范围
IFN-gamma: 15-2,000 pg/mL; IL-2: 2-200 pg/mL; IL-4: 4-500 pg/mL; IL-10: 30-4,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Streptavidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Streptavidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
运输条件
Wet ice
储存
2-8°C
蛋白名称
Th1/Th2
均相（无需洗涤）
Mouse
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
