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Invitrogen
Mouse pro-MMP-9 ELISA Kit
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产品规格
检测灵敏度
50 pg/mL
测定范围
50-18,000 pg/mL
检测样本类型/体积
{Plasma=2 µL, Serum=2 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
MMP-9
蛋白家族
Proteases Peptidases & Inhibitors
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse pro-MMP-9 ELISA quantitates Ms proMMP-9 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms proMMP-9.
原理方法
The Mouse proMMP-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AW743869, B/MMP9, Clg4b, MMP-9, Mmp9, pro-MMP-9
基因ID : (Mouse) 17395
基因符号 : Mmp9
蛋白别名 : 92 kDa gelatinase, 92 kDa type IV collagenase, 92kD gelatinase, 92kD type IV collagenase, 92kDa gelatinase, 92kDa type IV collagenase, collagenase type IVB, Gel B, Gelatinase B, GELB, matrix metalloproteinase 9, Matrix metalloproteinase-9, MMP-9, MMPs, Gelatinase-B, matrix metallo protease, Matrix metalloproteinase9, MMP
UniProt ID (Mouse) P41245
