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Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit
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![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/650/p38-phospho-tds.jpg?time=20240507 "Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit")
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Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit
Representative data for p38 MAPK (Phospho) [pT180/pY182] Multispecies InstantOne™ ELISA. HEK293 cells untreated (-) or treated with 2 µg/mL anisomycin for 30 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 38 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/150/p38-phospho-tds.jpg?time=20240507)
![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit (85-86022-11)](https://www.thermofisher.cn/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
p38 MAPK alpha
Target site-specificity
Phosphorylated Thr180, Phosphorylated Tyr182
均相（无需洗涤）
Human, Mouse
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p38 MAPK in cell lysates. Detection of rat p38 MAPK is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : Crk1, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAPK14, MXI2, p38, p38-alpha, p38a, p38ALPHA, p38MAPK, PRKM14, PRKM15, RK, SAPK2A
基因ID : (Human) 1432, (Mouse) 26416
基因符号 : MAPK14, Mapk14
蛋白别名 : CRK1, CSAID-binding protein, Csaids binding protein, cytokine suppressive anti-inflammatory drug binding protein, cytokine suppressive anti-inflammatory drug binding protein 1, Cytokine suppressive anti-inflammatory drug-binding protein, EC 2.7.11.24, ERK1, ERT2, MAP 14, MAP kinase 1, RP1-179N16.5, MAP kinase p38, MAP kinase p38alpha, MAPK1 antibody, MK14, OTTHUMP00000216563, OTTHUMP00000216566, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, mitogen activated protein kinase 14, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, p38 alpha, p38 MAP kinase, p38 MAP kinase alpha, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip, SAPK2a, Stress-activated protein kinase 2a, tRNA synthetase cofactor p38
UniProt ID (Human) Q16539, (Mouse) P47811
