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Invitrogen
Pregnanediol-3-Glucuronide (PDG) Competitive ELISA Kit
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产品规格
检测灵敏度
0.18 ng/mL
测定范围
0.391-50 ng/mL
检测样本类型/体积
{Fecal Extract=50 µL, Plasma=50 µL, Serum=50 µL, Supernatant=50 µL, Urine=10 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
6.4%
批内差异
4.1%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
Pregnanediol-3-Glucuronide
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Pregnanediol-3-Glucuronide (PDG) ELISA quantitates PDG in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.
原理方法
The Pregnanediol-3-Glucuronide (PDG) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure PDG present in diluted buffer samples and tissue culture media samples. A PDG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Pregnanediol-3-Glucuronide (PDG) is a natural human metabolite of Pregnanediol generated in the liver by UDP glucuonyltransferase. Glucuronidation is used to assist in the excretion of toxic substances, drugs or other substances that cannot be used as an energy source. Glucuronic acid is attached via a glycosidic bond to the substance, and the resulting glucuronide, which has much higher water solubility than the original substance, is eventually excreted by the kidneys.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : PDG, C27H44O8
