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Invitrogen
Rat IL1R2 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
18.75 pg/mL
Assay range
31.25-2000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
IL1R2
Species (tested)
Rat
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA quantitates IL-1R2 in serum and plasma.
Principle of the method
The Rat Interleukin 1 Receptor Type II (IL-1R2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Interleukin 1 Receptor Type II (IL-1R2) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Interleukin 1 Receptor Type II (IL-1R2). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Interleukin 1 Receptor Type II (IL-1R2) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Il-1r2, Il1r2, Il1rb
Gene ID : (Rat) 117022
Gene symbol : Il1r2
Protein Aliases : CD121 antigen-like family member B, CD121b, IL-1 type II receptor, IL-1R-2, IL-1R-beta, IL-1RT-2, IL-1RT2, ILR2, Interleukin-1 receptor beta, Interleukin-1 receptor type 2, Interleukin-1 receptor type II
UniProt ID (Rat) P43303
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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