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Invitrogen
Rat TIM-1 (HAVCR1) Rapid ELISA Kit
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产品规格
检测灵敏度
0.19 ng/mL
测定范围
0.31-20 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
KIM-1
均相（无需洗涤）
Rat
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Rat KIM-1 (Kidney Injury Molecule 1) ELISA quantitates KIM-1 in serum and plasma.
原理方法
The Rat Kidney Injury Molecule 1 (KIM-1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Kidney Injury Molecule 1 (KIM-1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Kidney Injury Molecule 1 (KIM-1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Kidney Injury Molecule 1 (KIM-1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Havcr1, KIM-1, Kim1
基因ID : (Rat) 286934
基因符号 : Havcr1
蛋白别名 : CD365, HAVcr-1, Hepatitis A virus cellular receptor 1 homolog, Kidney injury molecule 1, KIM-1, T cell immunoglobulin and mucin domain-containing protein 1, TIMD-1
UniProt ID (Rat) O54947
