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Invitrogen
Reverse T3 Competitive ELISA Kit
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产品规格
检测灵敏度
56.25 pg/mL
测定范围
93.75-6,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Reverse T3
蛋白家族
Thyroid hormone
均相（无需洗涤）
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The multispecies Reverse Triiodothyronine (rT3) ELISA quantitates rT3 in serum, plasma, and other biological fluids
原理方法:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Reverse Triiodothyronine (rT3) independent of species. A rT3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to rT3 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Reverse triiodothyronine (rT3) is a biologically inactive form of the thyroid hormone triiodothyronine (T3). rT3 is produced by the enzymatic deiodination of T4, primarily in peripheral tissues, and is mainly found in the liver and kidneys. Its primary function is to regulate the metabolism of T3 by blocking its action. The structure of rT3 is similar to that of T3, with the exception of the position of one iodine atom. This structural difference causes rT3 to be biologically inactive and unable to bind to thyroid hormone receptors. Despite its lack of direct physiological activity, rT3 levels can be used as a marker of thyroid hormone status and metabolic function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Reverse triiodothyronine
