Tango™ GPR44-bla U2OS cells contain the human G protein coupled receptor 44 (GPR44) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR44-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Indomethacin. In addition, Tango™ GPR44-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.
规格
编录号
NM_004778
检测条目
基于细胞的 β-arrestin 募集
细胞状态
分裂细胞
胞质尾区
GRPR GPCR 的尾区
检测方法
荧光
适用于(应用)
FRET:
人类蛋白质类
G 蛋白偶联受体 (GPCR)
种群状态
单细胞克隆起源
产品线
Tango™
读值
终点法
目标条目
CRTH2, GPR44
细胞系
U2OS
System Type
Tango™
Druggable Target
GPCR
内容与储存
Tango™ GPR44 (CRTH2)-bla U2OS 细胞置于干冰上运输,8 x 10^6 个细胞⁄瓶。接收后应立即储存于液氮中,或解冻后立即使用。