eBioscience™ TMB 溶液 (1X)

1x eBioscience TMB 溶液是一种灵敏的发色底物，以单一即用型溶液形式提供。TMB (3,3’,5,5’-四甲基联苯胺) 是辣根过氧化物酶 (HRP) 的底物，推荐用于 Ready-Set-Go!了解更多信息

货号	数量
00-4201-56	100 mL

货号 00-4201-56

价格（CNY)

470.00

Ends: 31-Dec-2025

625.00

共减 155.00 (25%)

添加至购物车

100 mL

价格（CNY)

470.00

Ends: 31-Dec-2025

625.00

共减 155.00 (25%)

添加至购物车

1x eBioscience TMB 溶液是一种灵敏的发色底物，以单一即用型溶液形式提供。TMB (3,3’,5,5’-四甲基联苯胺) 是辣根过氧化物酶 (HRP) 的底物，推荐用于 Ready-Set-Go! ELISA 试剂盒。

在酶促反应过程中，TMB 底物将产生蓝色，可以分光光度计上 650 nm 处读取。该动力学反应可以用 1 M 磷酸停止，导致颜色变化至可以在 450 nm 处读取的黄色。

已报告应用
ELISA

仅供科研使用。不可用于诊断程序。

检测方法比色法

产品线eBioscience™

底物属性化学底物

底物类型HRP（辣根过氧化物酶）

产品类型TMB 溶液

数量100 mL

Unit SizeEach

常见问题解答 (FAQ)

Are all your TMB substrates transparent and do they all turn blue after reacting with the HRP enzyme of an antibody?

No, we offer different TMB substrates for different applications.
We recommend the following TMB substrates for this particular application:
- eBioscience TMB Solution (1X) (Cat. No. 00-4201-56)
- (For higher sensitivity) 1-Step Ultra TMB-ELISA Substrate Solution (Cat. No. 34029)

What are the differences between TMB and Super AquaBlue?

TMB is a faster developing, stronger substrate that yields greater amplification/sensitivity/background. The use of an acid stop solution produces a yellow end product read at 450nm. Super AquaBlue is a slower developing blue/green substrate particularly useful for kinetic studies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What other reagents will I need to run an ELISA using the antibody pairs?

When working with our matched pairs or uncoated ELISA kits, please refer to the user guide provided with your antibody-matched pair for kit-specific guidance. When designing your own ELISA, please utilize our ELISA Builder tool for reagent selection as well as our ELISA Development and Optimization pages for futher guidance.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.